A 65-year-old Wallan man died after the bus he was driving crashed in Kilmore on Wednesday, May 22.
Emergency services said the school bus was travelling along Wandong Rd just after 3.30pm when the vehicle left the road and struck a tree.
There were about 30 children on the bus. Some sustained minor injuries, and but one suffered a serious but non-life-threatening injury.
The exact cause of the crash is being investigated.
The road is now open for all motorists after being closed due to investigations.
Anyone who witnessed the collision or has CCTV/dashcam footage or information is urged to contact Crime Stoppers on 1800 333 000 or www.crimestoppersvic.com.au