The decision by the Central Election Committee to exclude Heart of Moldova, part of the Patriotic Bloc (BEP), followed a court ruling limiting the party's activity for one year.
The justice ministry requested the restrictions following searches earlier this month on Heart of Moldova party members that led to allegations of voter bribery, illegal party financing and money laundering.
The electoral commission said in a statement that all candidates proposed by Heart of Moldova will be removed from the bloc's list of candidates, and gave the bloc 24 hours to adjust its list to meet the representation thresholds required by electoral law.
Sunday's poll could have sweeping consequences for Moldova, whose pro-European government has warned of extensive Russian-backed efforts to sway the vote and derail the country's bid to join the European Union.
Heart of Moldova said it was facing baseless attacks by the pro-EU government.
"This isn't justice but a final act of a dirty show orchestrated in advance by authorities with a single goal: to silence us," it said in a statement.
Thirty-six candidates have been struck from the ballot following the decision.
Party leader Irina Vlah was sanctioned by Latvia, Estonia and Poland this week on suspicion of assisting Russian interference.
The BEP says it wants friendship with Russia, "permanent neutrality" and a "state that serves the people, not officials".
In recent weeks, police have conducted hundreds of raids targeting illicit financing and alleged Russian-backed efforts to stoke unrest in an election campaign marred by widespread disinformation.
Polls indicate that the ruling Party of Action and Solidarity (PAS) could lose its majority as the BEP and other opponents court voters upset with high prices and the slow pace of reforms.
Having to govern in coalition could significantly hamper the PAS' effort to get Moldova into the EU by 2030, experts say.
with AP
