The day was organised by Australia Prays and was supported by churches in more than 90 locations around the country.
Rev John Payne, from the Uniting Church, hosted the Seymour event and opened with meditation and worship.
Representatives from each church then took turns to pray for topics that were important to them.
Puckapunyal chaplain Ricky Su shared his concerns for the welfare of army veterans.
Gil Dirnberger, from the Baptist Church, prayed for the planned referendum.
Jill Doherty, from the cafe church, prayed for the state government’s efforts to bring down the road toll.
Salvation Army captain Lauren Kirkham prayed for families in Seymour under stress from rising interest rates, homelessness and domestic violence.
Finally, Pastor Daniel Sanders from Central Church spoke about the shifting dynamics of the church throughout the world. He noted that in parts of Africa and Asia, the church was growing. However, in the Western world, the church was in decline.
Baptist church pastor Geoffrey Johnstone said that ultimately the things that united us were far more important than those things that divided us.
“Thirty per cent of my father’s generation were involved in church. Ten per cent of baby boomers identify as Christians. However, only three per cent of my son’s generation are interested,” he said.
“By the time we get to my grandchildren, it is less than one per cent who will ever see the inside of a church.”
Mr Sanders concluded the service with encouraging words.
“Ultimately, it's God’s church. So it will be interesting to see what plans He has for the future,” he said.